Thymosin Beta-4: Studies on tissue regeneration and repair processes

Source: Sosne G. et al., Frontiers in Pharmacology , 2022, 10.3389/fphar.2022.928133


Purpose of the study

To evaluate the effect of Thymosin Beta-4 (TB-500) on tissue healing, inflammation, and cell migration in models of trauma and inflammatory diseases.

TB-500 is a synthetic form of thymosin β4, a peptide that occurs naturally in cells and is involved in repair, angiogenesis, cell migration, and anti-inflammatory defense. The study aims to determine whether administering TB-500 can accelerate recovery and reduce the inflammatory response.


Study design

  • Type: preclinical, experimental study

  • Models: animal models (mice and rabbits) with induced wounds and inflammation

  • Application: local (gel, drops) and systemic (injection)

  • Doses: 0.5 – 5 mg/kg depending on the model

  • Duration: 7–21 days depending on the protocol

  • Measured parameters:

    • Wound healing rate

    • Angiogenesis and cell migration

    • Inflammatory cytokine levels

    • Histological analysis of tissues


Main results

Regeneration and healing

  • TB-500 significantly accelerates wound closure (up to 2× faster than control).

  • Stimulates angiogenesis and the formation of new capillaries around the damaged area.

  • Increases the expression of VEGF and actin-binding proteins, which support cell movement.

Anti-inflammatory effect

  • TB-500 reduces the levels of TNF-α, IL-1β, and IL-6 in affected tissues.

  • Reduces the infiltration of inflammatory cells (neutrophils, macrophages).

Cell migration and repair

  • Increases the mobility of fibroblasts and keratinocytes.

  • Improves the organization of the extracellular matrix and collagen fibers.

Safety

  • No toxic effects or changes in animal behavior were observed.

  • Good local tolerance in topical and systemic application.


Conclusions

  • TB-500 accelerates recovery and reduces inflammation in various injury models.

  • Stimulates cell migration and angiogenesis, promoting regeneration.

  • It shows an excellent safety profile and biological activity.

  • Suitable for examinations of muscle, tendon and skin injuries.


Restrictions

  • Preclinical models – no clinical data in humans.

  • An optimal dose for systemic administration has not been established.

  • No long-term effects of chronic damage have been observed.


Practical importance

  • TB-500 is a promising peptide for regenerative medicine.

  • Suitable for examinations of muscle and tendon injuries, wounds and inflammatory processes.

  • Need for clinical trials to confirm effects in humans.